IN VITRO FERTILIZATION

The in vitro Fertilization (FIV-ET) and its related techniques must be considered II° level techniques since they require a complex clinical and lab organization.
Fiv-ET consists in a lab culture of one or more oocytes and zoosperm and the following graft of the embryos in the uterus.
Fiv-ET indications are usually irreversible tubal injury, male medium factor, endometriosis, inexplicable sterility, immunological causes.
Collecting oocytes is a crucial step of this technique. Oocytes are taken by a vaginal probe while the patient is anaesthetized (local or total anaesthesia).
A staff is connected to the probe; a needle linked to a pump passes through the staff and arrives into the ovary where follicles are sucked. At this step risks are pelvic infection, pelvic pain, vaginal bleeding, abdominal bleeding.

Collected oocytes and partner’s spermatozoa are incubated together in the same vitro for 12-18 hours to form embryos. Then the embryos are incubated for about 24 hours. When cells are 4-8, say 48-72 hours from the collection of oocytes, embryos are transferred into intrauterine cavity. The rate of successful transfers varies from 25 to 35%. It depends on the age of the patient, embryonic quality, number of transferred embryos, and other non assessable parameters.

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